Mouse Anti-ATG5 Recombinant Antibody (clone 3C7) (CAT#: ZG-0038J)
This product is a mouse antibody that recognizes ATG5.
Specific Inquiry
Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
IF
Figure 1 Mouse Anti-ATG5 Recombinant Antibody (clone 3C7) in IF
Immunofluorescence analysis of Hela cell. 1, CREB-1 Polyclonal Antibody(red) was diluted at 1:200(4°C overnight). 1.ATG5 mouse Monoclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min)
IHC
Figure 2 Mouse Anti-ATG5 Recombinant Antibody (clone 3C7) in IHC
Immunohistochemical analysis of paraffin-embedded Human-breast tissue. 1.ATG5 Mouse Monoclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for Antibody retrieval(>98°C, 20min). 3, Secondary Antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary Antibody only.
IHC
Figure 3 Mouse Anti-ATG5 Recombinant Antibody (clone 3C7) in IHC
Immunohistochemical analysis of paraffin-embedded Rat-testis tissue. 1.ATG5 Mouse Monoclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for Antibody retrieval(>98°C, 20min). 3, Secondary Antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary Antibody only.
WB
Figure 4 Mouse Anti-ATG5 Recombinant Antibody (clone 3C7) in WB
Western blot analysis of Hela Cell Lysate using ATG5 Mouse mAb diluted at 1:10000
Specifications
- Immunogen
- Recombinant Protein of ATG5
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human ATG5
- Species Reactivity
- Human, Mouse, Rat
- Clone
- 3C7
- Applications
- IF/ICC, WB, IHC-P, IP
Product Property
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Immunofluorescence/Immunocytochemistry, Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation.
Target
- Alternative Names
- Autophagy Related 5; Apoptosis-Specific Protein; APG5-LIKE; APG5L; ASP; ATG5 Autophagy Related 5 Homolog (S. Cerevisiae); APG5 (Autophagy 5, S. Cerevisiae)-Like; APG5 Autophagy 5-Like (S. Cerevisiae)
- Gene ID
- 9474
- UniProt ID
- Q9H1Y0
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Customer Reviews and Q&As
Customer Review
Q&As
Perpetual Excellence
Because we have consistently ordered this antibody throughout multiple batches, we are confident that it will perform as expected in investigations.
Robust Function
Because it performs well in a variety of experimental settings, this antibody is a good option for studies.
Not in a Determinative Entirety
Our results were much more precise because our Western blots showed no non-specific binding.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# ZG-0038J, RRID: AB_3112060)
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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