Mouse Anti-ATG5 Recombinant Antibody (clone 3C7)
CAT#: ZG-0038J
This product is a mouse antibody that recognizes ATG5.
Tested Data
Gene Expression
IF
Figure 1 Mouse Anti-ATG5 Recombinant Antibody (clone 3C7) in IF
Immunofluorescence analysis of Hela cell. 1, CREB-1 Polyclonal Antibody(red) was diluted at 1:200(4°C overnight). 1.ATG5 mouse Monoclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min)
IHC
Figure 2 Mouse Anti-ATG5 Recombinant Antibody (clone 3C7) in IHC
Immunohistochemical analysis of paraffin-embedded Human-breast tissue. 1.ATG5 Mouse Monoclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for Antibody retrieval(>98°C, 20min). 3, Secondary Antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary Antibody only.
IHC
Figure 3 Mouse Anti-ATG5 Recombinant Antibody (clone 3C7) in IHC
Immunohistochemical analysis of paraffin-embedded Rat-testis tissue. 1.ATG5 Mouse Monoclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for Antibody retrieval(>98°C, 20min). 3, Secondary Antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary Antibody only.
WB
Figure 4 Mouse Anti-ATG5 Recombinant Antibody (clone 3C7) in WB
Western blot analysis of Hela Cell Lysate using ATG5 Mouse mAb diluted at 1:10000
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Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to centrosome.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/42973/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human stomach
Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/34432/ihc_selected.jpg
Normal Tissue
Figure 3 Lymph node
Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/34432/64112_A_9_8.jpg
Normal Tissue
Figure 4 Thyroid gland
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/34432/64112_B_2_5.jpg
Normal Tissue
Figure 5 Colon
Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/34432/64112_A_9_3.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Leydig cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/34432/64112_A_4_6.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000057663-ATG5
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Specifications
- Immunogen
- Recombinant Protein of ATG5
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human ATG5
- Species Reactivity
- Human, Mouse, Rat
- Clone
- 3C7
- Applications
- IF, ICC, WB, IHC-P, IP
Product Property
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Immunofluorescence/Immunocytochemistry, Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation.
Target
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Perpetual Excellence
Because we have consistently ordered this antibody throughout multiple batches, we are confident that it will perform as expected in investigations.
Robust Function
Because it performs well in a variety of experimental settings, this antibody is a good option for studies.
Not in a Determinative Entirety
Our results were much more precise because our Western blots showed no non-specific binding.
Q&As
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What are isotype regulates, and why do they matter?
A: Isotype controls are antibodies of the same class and subclass but with irrelevant specificity, used to gauge non-specific binding.
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How does buffer composition influence antibody binding?
A: Buffer composition, including pH and ionic strength, can impact antibody-antigen interactions; optimization is essential for best results.
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Can I share antibody aliquots with another colleague?
A: Share antibody aliquots with proper tracking of lot numbers and handling conditions to ensure consistency.
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# ZG-0038J, RRID: AB_3112060)
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "ATG5"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0058MZ | Chicken Anti-ATG5 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
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| MOR-0283 | Hi-Affi™ Rabbit Anti-ATG5 Recombinant Antibody (clone DS283AB) | WB, IHC, ICC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY23 | AbPlus™ Anti-ATG5 Magnetic Beads (3C7) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC76 | CytoStream™ Rabbit Anti-ATG5 Recombinant Antibody (VS13-YC76) | WB, IHC-P, ICC, IF, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY591 | Anti-ATG5 Immunohistochemistry Kit | IHC | |
| VS-0525-XY592 | Anti-Mouse ATG5 Immunohistochemistry Kit | IHC |
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